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Next-generation RNA sequencing unlocks the discovery of novel candidate biomarkers for diagnostic and therapeutic applications, but reproducibility is a key challenge in biomarker discovery programs. Integrating multi-faceted quality control (QC) steps can improve design decisions and enhance the confidence and reliability of results. This webinar will demonstrate how a comprehensive QC framework supports a disease-agnostic biomarker discovery program to identify biologically relevant candidate biomarkers of complex disease and how this framework can be adapted for total RNA sequencing of whole blood, plasma, and serum samples.